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XIA Yanzhi, WAN Xuedong, DUAN Qiuhong, HE Shansu, WANG Ximing
《医学前沿(英文)》 2007年 第1卷 第2期 页码 200-206 doi: 10.1007/s11684-007-0038-y
Relationship between reactive oxygen species and sodium-selenite-induced DNA damage in HepG2 cells
ZOU Yunfeng, NIU Piye, GONG Zhiyong, YANG Jin, YUAN Jing, WU Tangchun, CHEN Xuemin
《医学前沿(英文)》 2007年 第1卷 第3期 页码 327-332 doi: 10.1007/s11684-007-0063-x
转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化 Article
Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš
《工程(英文)》 2024年 第32卷 第1期 页码 58-69 doi: 10.1016/j.eng.2023.09.019
Hepatocyte nuclear factor 1 alpha (HNF1A), hepatocyte nuclear factor 4 alpha (HNF4A), and forkhead box protein A2 (FOXA2) are key transcription factors that regulate a complex gene network in the liver, creating a regulatory transcriptional loop. The Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase genes. Our in silico analysis of HNF1A, HNF4A, and FOXA2 binding to the 10 candidate glyco-genes studied in this work confirms a significant enrichment of these transcription factors specifically in the liver. Our previous studies identified HNF1A as a master regulator of fucosylation, glycan branching, and galactosylation of plasma glycoproteins. Here, we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan phenotype. We used the state-of-the-art clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) molecular tool for the downregulation of the HNF1A, HNF4A, and FOXA2 genes in HepG2 cells—a human liver cancer cell line. The results show that the downregulation of all three genes individually and in pairs affects the transcriptional activity of many glyco-genes, although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan structures. The effect is better seen as an overall change in the total HepG2 N-glycome, primarily due to the extension of biantennary glycans. We propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan structure. We also propose a model showing feedback loops with the mutual activation of HNF1A–FOXA2 and HNF4A–FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.
关键词: Clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) Epigenetics Hepatocyte nuclear factor 1 alpha (HNF1A) Hepatocyte nuclear factor 4 alpha (HNF4A) Forkhead box protein A2 (FOXA2) N-glycosylation HepG2 cells
GID complex regulates the differentiation of neural stem cells by destabilizing TET2
《医学前沿(英文)》 doi: 10.1007/s11684-023-1007-9
关键词: TET2 GID complex neural stem cells differentiation of neurons
Transplantation of placenta-derived mesenchymal stem cells in type 2 diabetes: a pilot study
null
《医学前沿(英文)》 2011年 第5卷 第1期 页码 94-100 doi: 10.1007/s11684-011-0116-z
Mesenchymal stem cells (MSC) have been used in clinical trials for severe diabetes, a chronic disease with high morbidity and mortality. Bone marrow is the traditional source of human MSC, but human term placenta appears to be an alternative and more readily available source. Here, the therapeutic effect of human placenta-derived MSC (PD-MSC) was studied in type 2 diabetes patients with longer duration, islet cell dysfunction, high insulin doses as well as poor glycemic control in order to evaluate the safety, efficacy and feasibility of PD-MSC treatment in type 2 diabetes (T2D). Ten patients with T2D received three intravenous infusions of PDSC, with one month interval of infusion. The total number of PDSC for each patient was (1.22–1.51) × 106/kg, with an average of 1.35 × 106/kg. All of the patients were followed up after therapy for at least 3 months. A daily mean dose of insulin used in 10 patients was decreased from 63.7?±?18.7 to 34.7?±?13.4 IU (P<0.01), and the C-peptide level was increased from 4.1?±?3.7 ng/mL to 5.6?±?3.8 ng/mL (P<0.05) respectively after therapy. In 4 of 10 responders their insulin doses reduced more than 50% after infusion. The mean levels of insulin and C-peptide at each time point in a total of 10 patients was higher after treatment (P<0.05). No fever, chills, liver damage and other side effects were reported. The renal function and cardiac function were improved after infusion. The results obtained from this pilot clinical trial indicate that transplantation of PD-MSC represents a simple, safe and effective therapeutic approach for T2D patients with islet cell dysfunction. Further large-scale, randomized and well-controlled clinical studies will be required to substantiate these observations.
FAN Xiaodong, HAN Ruifa
《医学前沿(英文)》 2007年 第1卷 第4期 页码 377-380 doi: 10.1007/s11684-007-0073-8
SALL4 maintains self-renewal of porcine pluripotent stem cells through downregulation of OTX2
Ning WANG, Sile WANG, Yaxian WANG, Yuanxing CAI, Fan YANG, Huayan WANG
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 81-92 doi: 10.15302/J-FASE-2017180
Sall4 as one of the spalt family members contains several alternative splicing variants, which are differentially expressed and has a key role in maintaining pluripotent stem cells. However, the molecular features and function of SALL4 have not been well elucidated in porcine induced pluripotent stem cells (piPSCs). In this study, we identified splice variants and found two splicing variants through analysis of the porcine transcriptome data derived from piPSCs. SALL4A was only detected in piPSCs but SALL4B was globally expressed in porcine tissues and piPSCs. The level of SALL4B was significantly reduced when piPSCs differentiation occurred, however, the expression of SALL4A was not affected, indicating that SALL4B may be essential for the maintenance of piPSCs self-renewal. Overexpression of SALL4A and SALL4B in PEF cells could significantly stimulated expression of endogenous pluripotent genes, when SALL4B significantly promoted OCT4 expression. Conversely, SALL4A significantly promoted KLF4 expression. Additionally, both SALL4A and SALL4B could repress promoter activity in a dose-dependent manner. Conversely, OTX2 also negatively regulated SALL4 expression. These observations indicate that a negative feedback regulatory mechanism may exist between SALL4 and OTX2, which is useful for the maintenance of the self-renewal of piPSCs.
《医学前沿(英文)》 doi: 10.1007/s11684-023-0999-5
关键词: thyroid carcinoma mesenchymal stem cell extracellular vesicle GTF2I FAT1 CDK4
Wen YAN MD, Min FENG MD, Pei-Hua WANG MD, Dao-Wen WANG MD,
《医学前沿(英文)》 2010年 第4卷 第2期 页码 225-228 doi: 10.1007/s11684-010-0003-z
关键词: bradykinin vascular smooth muscle cells signal transduction pathways
《医学前沿(英文)》 2023年 第17卷 第4期 页码 781-795 doi: 10.1007/s11684-023-0986-x
关键词: dry eye disease glycolytic reprogramming pyroptosis inflammation 2-DG
《医学前沿(英文)》 2022年 第16卷 第4期 页码 637-650 doi: 10.1007/s11684-021-0864-3
关键词: apigenin aminoglycosides ototoxicity oxidative stress Nrf2 signaling pathway
孙云,林舒平,李伟,程世清,张运祥,刘一鸣,刘玮
《工程(英文)》 2017年 第3卷 第4期 页码 452-459 doi: 10.1016/J.ENG.2017.04.020
本文总结了Cu(In, Ga)Se2 (CIGS) 电池中掺杂碱金属的发展历史和一些重要成果,综述了碱金属掺杂方式对CIGS 吸收层及器件性能的影响。通过分析由(NaF+KF)-PDT 导致的CIGS 表面结构及电学性能的改变,我们提出并解释了如下几个问题:①在低温沉积CIGS 薄膜过程中,Na 促进了CuInSe2的优先形成,转型为施主缺陷
Orlistat induces ferroptosis-like cell death of lung cancer cells
《医学前沿(英文)》 2021年 第15卷 第6期 页码 922-932 doi: 10.1007/s11684-020-0804-7
Enhancement of open circuit voltage in organic solar cells by doping a fluorescent red dye
Qing LI, Junsheng YU, Yue ZANG, Nana WANG, Yadong JIANG
《能源前沿(英文)》 2012年 第6卷 第2期 页码 179-183 doi: 10.1007/s11708-012-0177-y
关键词: organic solar cells (OSCs) open circuit voltage fluorescent dye doping 4-(dicyanomethylene)-2-t-butyl-6-(1 1 7 7-tetramethyljulolidyl-9-enyl)-4H-pyran (DCJTB)
Andrew Best,Katherine James,Gerald Hysenaj,Alison Tyson-Capper,David J. Elliott
《化学科学与工程前沿(英文)》 2016年 第10卷 第2期 页码 186-195 doi: 10.1007/s11705-015-1540-4
关键词: RNA splicing gene expression breast cancer DNA damage CHK1
标题 作者 时间 类型 操作
Inhibition of protein kinase B by Palmitate in the insulin signaling of HepG2 cells and the preventive
XIA Yanzhi, WAN Xuedong, DUAN Qiuhong, HE Shansu, WANG Ximing
期刊论文
Relationship between reactive oxygen species and sodium-selenite-induced DNA damage in HepG2 cells
ZOU Yunfeng, NIU Piye, GONG Zhiyong, YANG Jin, YUAN Jing, WU Tangchun, CHEN Xuemin
期刊论文
转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化
Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš
期刊论文
Transplantation of placenta-derived mesenchymal stem cells in type 2 diabetes: a pilot study
null
期刊论文
Study of recombinant human IFN-α-2b bacilli Calmette–Guerin activated killer cells and against bladder
FAN Xiaodong, HAN Ruifa
期刊论文
SALL4 maintains self-renewal of porcine pluripotent stem cells through downregulation of OTX2
Ning WANG, Sile WANG, Yaxian WANG, Yuanxing CAI, Fan YANG, Huayan WANG
期刊论文
Extracellular vesicle-carried GTF2I from mesenchymal stem cells promotes the expression of tumor-suppressive
期刊论文
Effect of bradykinin on bradykinin-B2 receptor in rat aortic vascular smooth muscle cells and the involved
Wen YAN MD, Min FENG MD, Pei-Hua WANG MD, Dao-Wen WANG MD,
期刊论文
Hyperosmolarity promotes macrophage pyroptosis by driving the glycolytic reprogramming of corneal epithelial cells
期刊论文
Apigenin alleviates neomycin-induced oxidative damage via the Nrf2 signaling pathway in cochlear haircells
期刊论文
Enhancement of open circuit voltage in organic solar cells by doping a fluorescent red dye
Qing LI, Junsheng YU, Yue ZANG, Nana WANG, Yadong JIANG
期刊论文